Microbial community dynamics in replicate anaerobic digesters exposed sequentially to increasing organic loading rate, acidosis, and process recovery
Identifieur interne : 000251 ( Main/Exploration ); précédent : 000250; suivant : 000252Microbial community dynamics in replicate anaerobic digesters exposed sequentially to increasing organic loading rate, acidosis, and process recovery
Auteurs : Xavier Goux [Luxembourg (pays), France] ; Magdalena Calusinska [Luxembourg (pays)] ; Sébastien Lemaigre [Luxembourg (pays)] ; Martyna Marynowska [Luxembourg (pays)] ; Michael Klocke [Allemagne] ; Thomas Udelhoven [Allemagne] ; Emile Benizri [France] ; Philippe Delfosse [Luxembourg (pays)]Source :
- Biotechnology for Biofuels [ 1754-6834 ] ; 2015.
Abstract
Volatile fatty acid intoxication (acidosis), a common process failure recorded in anaerobic reactors, leads to drastic losses in methane production. Unfortunately, little is known about the microbial mechanisms underlining acidosis and the potential to recover the process. In this study, triplicate mesophilic anaerobic reactors of 100 L were exposed to acidosis resulting from an excessive feeding with sugar beet pulp and were compared to a steady-state reactor.
Stable operational conditions at the beginning of the experiment initially led to similar microbial populations in the four reactors, as revealed by 16S rRNA gene T-RFLP and high-throughput amplicon sequencing.
In this study we show that once the replicate reactors are confronted with sub-optimal conditions, their microbial populations start to evolve differentially. Furthermore the alterations of commonly used microbial parameters to monitor the process, such as richness, evenness and diversity indices were unsuccessful to predict the process failure. At the same time, we tentatively propose the replacement of the dominant
The online version of this article (doi:10.1186/s13068-015-0309-9) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/s13068-015-0309-9
PubMed: 26288654
PubMed Central: 4539856
Affiliations:
- Allemagne, France, Luxembourg (pays)
- Brandebourg, Grand Est, Lorraine (région)
- Potsdam, Vandœuvre-lès-Nancy
- Université de Lorraine
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Le document en format XML
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<author><name sortKey="Delfosse, Philippe" sort="Delfosse, Philippe" uniqKey="Delfosse P" first="Philippe" last="Delfosse">Philippe Delfosse</name>
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<series><title level="j">Biotechnology for Biofuels</title>
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>Volatile fatty acid intoxication (acidosis), a common process failure recorded in anaerobic reactors, leads to drastic losses in methane production. Unfortunately, little is known about the microbial mechanisms underlining acidosis and the potential to recover the process. In this study, triplicate mesophilic anaerobic reactors of 100 L were exposed to acidosis resulting from an excessive feeding with sugar beet pulp and were compared to a steady-state reactor.</p>
</sec>
<sec><title>Results</title>
<p>Stable operational conditions at the beginning of the experiment initially led to similar microbial populations in the four reactors, as revealed by 16S rRNA gene T-RFLP and high-throughput amplicon sequencing. <italic>Bacteroidetes</italic>
and <italic>Firmicutes</italic>
were the two dominant phyla, and although they were represented by a high number of operational taxonomic units, only a few were dominant. Once the environment became deterministic (selective pressure from an increased substrate feeding), microbial populations started to diverge between the overfed reactors. Interestingly, most of bacteria and archaea showed redundant functional adaptation to the changing environmental conditions. However, the dominant <italic>Bacteroidales</italic>
were resistant to high volatile fatty acids content and low pH. The severe acidosis did not eradicate archaea and a clear shift in archaeal populations from acetotrophic to hydrogenotrophic methanogenesis occurred in the overfed reactors. After 11 days of severe acidosis (pH 5.2 ± 0.4), the process was quickly recovered (restoration of the biogas production with methane content above 50 %) in the overfed reactors, by adjusting the pH to around 7 using NaOH and NaHCO<sub>3</sub>
.</p>
</sec>
<sec><title>Conclusions</title>
<p>In this study we show that once the replicate reactors are confronted with sub-optimal conditions, their microbial populations start to evolve differentially. Furthermore the alterations of commonly used microbial parameters to monitor the process, such as richness, evenness and diversity indices were unsuccessful to predict the process failure. At the same time, we tentatively propose the replacement of the dominant <italic>Methanosaeta</italic>
sp. in this case by <italic>Methanoculleus</italic>
sp., to be a potential warning indicator of acidosis.</p>
</sec>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1186/s13068-015-0309-9) contains supplementary material, which is available to authorized users.</p>
</sec>
</div>
</front>
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<author><name sortKey="Pelletier, Da" uniqKey="Pelletier D">DA Pelletier</name>
</author>
<author><name sortKey="Lane, Dj" uniqKey="Lane D">DJ Lane</name>
</author>
</analytic>
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<author><name sortKey="Pruesse, E" uniqKey="Pruesse E">E Pruesse</name>
</author>
<author><name sortKey="Schweer, T" uniqKey="Schweer T">T Schweer</name>
</author>
<author><name sortKey="Peplies, J" uniqKey="Peplies J">J Peplies</name>
</author>
<author><name sortKey="Quast, C" uniqKey="Quast C">C Quast</name>
</author>
<author><name sortKey="Horn, M" uniqKey="Horn M">M Horn</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Grosskopf, R" uniqKey="Grosskopf R">R Grosskopf</name>
</author>
<author><name sortKey="Janssen, Ph" uniqKey="Janssen P">PH Janssen</name>
</author>
<author><name sortKey="Liesack, W" uniqKey="Liesack W">W Liesack</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lueders, T" uniqKey="Lueders T">T Lueders</name>
</author>
<author><name sortKey="Friedrich, M" uniqKey="Friedrich M">M Friedrich</name>
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<affiliations><list><country><li>Allemagne</li>
<li>France</li>
<li>Luxembourg (pays)</li>
</country>
<region><li>Brandebourg</li>
<li>Grand Est</li>
<li>Lorraine (région)</li>
</region>
<settlement><li>Potsdam</li>
<li>Vandœuvre-lès-Nancy</li>
</settlement>
<orgName><li>Université de Lorraine</li>
</orgName>
</list>
<tree><country name="Luxembourg (pays)"><noRegion><name sortKey="Goux, Xavier" sort="Goux, Xavier" uniqKey="Goux X" first="Xavier" last="Goux">Xavier Goux</name>
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<name sortKey="Calusinska, Magdalena" sort="Calusinska, Magdalena" uniqKey="Calusinska M" first="Magdalena" last="Calusinska">Magdalena Calusinska</name>
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<name sortKey="Lemaigre, Sebastien" sort="Lemaigre, Sebastien" uniqKey="Lemaigre S" first="Sébastien" last="Lemaigre">Sébastien Lemaigre</name>
<name sortKey="Marynowska, Martyna" sort="Marynowska, Martyna" uniqKey="Marynowska M" first="Martyna" last="Marynowska">Martyna Marynowska</name>
</country>
<country name="France"><region name="Grand Est"><name sortKey="Goux, Xavier" sort="Goux, Xavier" uniqKey="Goux X" first="Xavier" last="Goux">Xavier Goux</name>
</region>
<name sortKey="Benizri, Emile" sort="Benizri, Emile" uniqKey="Benizri E" first="Emile" last="Benizri">Emile Benizri</name>
<name sortKey="Benizri, Emile" sort="Benizri, Emile" uniqKey="Benizri E" first="Emile" last="Benizri">Emile Benizri</name>
<name sortKey="Goux, Xavier" sort="Goux, Xavier" uniqKey="Goux X" first="Xavier" last="Goux">Xavier Goux</name>
</country>
<country name="Allemagne"><region name="Brandebourg"><name sortKey="Klocke, Michael" sort="Klocke, Michael" uniqKey="Klocke M" first="Michael" last="Klocke">Michael Klocke</name>
</region>
<name sortKey="Udelhoven, Thomas" sort="Udelhoven, Thomas" uniqKey="Udelhoven T" first="Thomas" last="Udelhoven">Thomas Udelhoven</name>
</country>
</tree>
</affiliations>
</record>
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